About us

By interfacing advanced bioengineering strategies and stem cell biology, the overarching goal of the Lutolf Laboratory is to uncover mechanisms of stem cell fate regulation; knowledge that will contribute to better ways to grow stem cells in culture and use them for various applications. A major current focus of the lab is on coaxing stem cells in vitro into miniature, organ-mimicking constructs, termed ‘organoids’, by exposing them to well-controlled artificial signaling microenvironments. The lab’s ambition is to achieve organoid development in a more reproducible and ultimately predictable manner by ‘guiding’ stem cell-based self-organization. Beyond contributing to a better understanding of key principles of multicellular self-organization, this research should open up exciting prospects for drug discovery and regenerative medicine.

Selected publications

Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M.E., Ordonez-Moran, P., Clevers, H., Lutolf, M.P.*, Designer matrices for intestinal stem cell and organoid culture, Nature, 539, 560-564 (2016)

Ranga, A., Girgin, M., Meinhardt, A., Eberle, D., Caiazzo, M., Tanaka, E.M., Lutolf, M.P.*, Neural tube morphogenesis in synthetic 3D microenvironments, PNAS, E6831–E6839, doi: 10.1073/pnas.1603529113 doi: 10.1073/pnas.1603529113 (2016)

Vannini, N., Girotra, M., Naveiras, O., Nikitin, G., Campos, V., Giger, S., Roch, A., Auwerx, J., Lutolf, M.P.*, Specification of haematopoietic stem cell fate via modulation of mitochondrial activity, Nature Communications, 7. 13125. doi:10.1038/ncomms13125 (2016)

Brandenberg, N., Lutolf, M.P.*, In situ patterning of microfluidic networks in 3D cell-laden hydrogels, Advanced Materials, 28(34):7450-6. doi: 10.1002/adma.201601099 (2016)

Caiazzo, M., Okawa, Y., Ranga, A., Piersigilli, A., Tabata,Y., Lutolf, M.P.*, Defined three-dimensional microenvironments boost induction of pluripotency, Nature Materials, 15, 344–352 (2016)

Gjorevski, N.*, Ranga, A., Lutolf, M.P.*, Bioengineering approaches to guide stem cell-based organogenesis, Development 141 (9), 1794-1804 (2014)

Ranga, A., Gobaa, S., Mosiewicz, K.A., Okawa, Y., Negro, A., Lutolf, M.P.*, 3D niche microarrays for systems-level analyses of cell fate, Nature Communications, 5, 4324 (2014)

Mosiewicz, K.A., Kolb, L., van der Vlies, A.J., Martino, M.M., Lienemann, P.S., Hubbell, J.A., Ehrbar, M., Lutolf, M.P.*, In situ cell manipulation through enzymatic hydrogel photopatterning, Nature Materials, 12 (11), 1072-1078 (2014)

Roccio, M., Schmitter, D., Knobloch, M., Okawa, Y., Sage, D., and Lutolf M.P.*, Predicting stem cell fate changes by differential cell cycle progression patterns, Development, 140, 459-470 (2013)

Gobaa, S., Hoehnel, S., Roccio, M., Negro, A., Kobel, S., and Lutolf M.P.*, Artificial niche microarrays for probing single stem cell fate in high throughput, Nature Methods, 8(11):949-55 (2011)

Gilbert, P.M., Havenstrite, K.L., Magnusson, K.E.G., Sacco, A., Leonardi, N.A., Kraft, P., Nguyen, N.K., Thrun, S., Lutolf, M.P. and Blau, H.M.*, Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture, Science, 329(5995):1078-1081 (2010)

Lutolf, M.P.*, Gilbert, P.M., and Blau, H.M.*, Designing materials to direct stem-cell fate, Nature, 462, 433-441 (2009)

 

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